![Ligand Activation: Human TLR9 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26280] - LR9/NF-kB SEAPorterTM HEK 293 cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amount of CpG for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. A. CpG-mediated TLR9 activation, as measured by SEAP activity, was increased in a dose dependent manner. B. The values from (A) were used to determine the EC50 of CpG. The EC50, or the half maximal activity concentration, represents the concentration of CpG that was required for 50% activation of TLR9 as measured by SEAP activity. Data Summary: CpG activated the cell line in a dose response manner, of which EC50 was measured as 4.401 ug/ml.](http://www.bioprodhub.com/system/product_images/cel_products/5/sub_2/10745_Human-TLR9-NF-kB-SEAP---(SEAPorter(TM))-Stable-Reporter-Cell-Line-Ligand-Activation-NBP2-26280-img0003.jpg)
Ligand Activation: Human TLR9 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26280] - LR9/NF-kB SEAPorterTM HEK 293 cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amount of CpG for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. A. CpG-mediated TLR9 activation, as measured by SEAP activity, was increased in a dose dependent manner. B. The values from (A) were used to determine the EC50 of CpG. The EC50, or the half maximal activity concentration, represents the concentration of CpG that was required for 50% activation of TLR9 as measured by SEAP activity. Data Summary: CpG activated the cell line in a dose response manner, of which EC50 was measured as 4.401 ug/ml.